Journal of the American Academy of Dermatology
Volume 59, Issue 5 , Pages 814-821 , November 2008

Sampling of melanocytic nevi for research purposes: A prospective, pilot study to determine effect on diagnosis

  • Scott R. Florell, MD

      Affiliations

    • The Melanoma Program, Huntsman Cancer Institute, University of Utah, Salt Lake City, Utah
    • Department of Dermatology, University of Utah, Salt Lake City, Utah
    • Corresponding Author InformationReprint requests: Scott R. Florell, MD, Department of Dermatology, University of Utah Health Sciences Center, 4B454 SOM, Salt Lake City, UT 84132.
    • ,
  • Bruce R. Smoller, MD

      Affiliations

    • Department of Pathology, University of Arkansas for Medical Sciences, Little Rock, Arkansas
    • ,
  • Kenneth M. Boucher, PhD

      Affiliations

    • Department of Oncological Sciences, University of Utah, Salt Lake City, Utah
    • ,
  • Douglas Grossman, MD, PhD

      Affiliations

    • The Melanoma Program, Huntsman Cancer Institute, University of Utah, Salt Lake City, Utah
    • Department of Dermatology, University of Utah, Salt Lake City, Utah
    • Department of Oncological Sciences, University of Utah, Salt Lake City, Utah
    • ,
  • Ronald M. Harris, MD, MBA

      Affiliations

    • The Melanoma Program, Huntsman Cancer Institute, University of Utah, Salt Lake City, Utah
    • Department of Dermatology, University of Utah, Salt Lake City, Utah
    • ,
  • Glen M. Bowen, MD

      Affiliations

    • The Melanoma Program, Huntsman Cancer Institute, University of Utah, Salt Lake City, Utah
    • Department of Dermatology, University of Utah, Salt Lake City, Utah
    • ,
  • Sancy A. Leachman, MD, PhD

      Affiliations

    • The Melanoma Program, Huntsman Cancer Institute, University of Utah, Salt Lake City, Utah
    • Department of Dermatology, University of Utah, Salt Lake City, Utah

References 

  1. Feldman MY. Reactions of nucleic acids and nucleoproteins with formaldehyde. Prog Nucleic Acid Res Mol Biol. 1973;13:1–49
  2. Moller K, Rinke J, Ross A, Buddle G, Brimacombe R. The use of formaldehyde in RNA-protein cross-linking studies with ribosomal subunits from Escherichia coli. Eur J Biochem. 1977;76:175–187
  3. Werner M, Chott A, Fabiano A, Battifora H. Effect of formalin tissue fixation and processing on immunohistochemistry. Am J Surg Pathol. 2000;24:1016–1019
  4. Florell SR, Coffin CM, Holden JA, Zimmermann JW, Gerwels JW, Summers BK, et al. Preservation of RNA for functional genomic studies: a multidisciplinary tumor bank protocol. Mod Pathol. 2001;14:116–128
  5. Kendall BS, Ronnett BM, Isacson C, Cho KR, Hedrick L, Diener-West M, et al. Reproducibility of the diagnosis of endometrial hyperplasia, atypical hyperplasia, and well-differentiated carcinoma. Am J Surg Pathol. 1998;22:1012–1019
  6. Landis JR, Koch GG. The measurement of observer agreement for categorical data. Biometrics. 1977;33:159–174
  7. Bolognia JL, Lin A, Shapiro PE. The significance of eccentric foci of hyperpigmentation ('small dark dots') within melanocytic nevi. Analysis of 59 cases. Arch Dermatol. 1994;130:1013–1017
  8. Corona R, Mele A, Amini M, De Rosa G, Coppola G, Piccardi P, et al. Interobserver variability on the histopathologic diagnosis of cutaneous melanoma and other pigmented skin lesions. J Clin Oncol. 1996;14:1218–1223
  9. Duncan LM, Berwick M, Bruijn JA, Byers HR, Mihm MC, Barnhill RL. Histopathologic recognition and grading of dysplastic melanocytic nevi: an interobserver agreement study. J Invest Dermatol. 1993;100:318S–321S
  10. Farmer ER, Gonin R, Hanna MP. Discordance in the histopathologic diagnosis of melanoma and melanocytic nevi between expert pathologists. Hum Pathol. 1996;27:528–531
  11. Florell SR, Boucher KM, Leachman SA, Azmi F, Harris RM, Malone JC, et al. Histopathologic recognition of involved margins of lentigo maligna excised by staged excision: an interobserver comparison study. Arch Dermatol. 2003;139:595–604
  12. Wechsler J, Bastuji-Garin S, Spatz A, Bailly C, Cribier B, Andrac-Meyer L, et al. Reliability of the histopathologic diagnosis of malignant melanoma in childhood. Arch Dermatol. 2002;138:625–628

 Supported by grants from The Skin Cancer Foundation (to S. R. F.), the Dermatology Foundation Leaders Society Dermatologist Investigator Research Fellowship and Clinical Career Development Award (to S. R. F.); National Institutes of Health grants K23 RR17525-01 (to S. R. F.) and R01 AR50102 (to D. G.), Doris Duke Charitable Foundation (to S. A. L.), Fellowship-To-Faculty Transition Award from the University of Utah funded in part by the Howard Hughes Medical Institute (to S. A. L.), The Huntsman Cancer Foundation (to S. A. L., D. G.), the Tom C. Mathews Jr. Familial Melanoma Research Endowment, Huntsman General Clinical Research Center Public Health Service grant (MO1 RR00064), National Cancer Institute (NCI) Cancer Center Support grants 5P30CA420-14, and the Utah Cancer Registry, funded by Contract # N01-PC-35141 from the NCI with additional support from the Utah Department of Health and the University of Utah.

 Conflicts of interest: None declared.

PII: S0190-9622(08)00911-0

doi: 10.1016/j.jaad.2008.07.020

Journal of the American Academy of Dermatology
Volume 59, Issue 5 , Pages 814-821 , November 2008

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